Today me and Zosia got a plasmid each to transform competent bacteria with. We would need them later for transfections. I really liked it because microbial cultures are something I had done quite a lot so I felt confident. For the whole transformation it was mainly following a protocol, but I still did something else.
Neurons and fibroblasts weren't dense enough for passaging today yet, so I will split them tomorrow and transfect on Saturday.
I am also in charge of a live imaging. I have been taking pictures of live cells every two hours since 12.15pm and in 30min I will set the microscope on automatic imaging. Hopefully it won't break this time, with a new light bulb!
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